Advances in CRISPR-Cas9 Tools and Applications for Toxicologists
SOT Virtual CE Course: SR01
Chair(s): Cheryl Rockwell, Michigan State University; and Elena Demireva, Michigan State University.
Continuing Education Committee
Mechanisms Specialty Section; Molecular and Systems Biology Specialty Section
CRISPR-Cas-based technologies have revolutionized science by significantly decreasing the time, money, expertise, and labor required to implement gene editing. Thus, this approach is becoming routine in many laboratories as a facile method to alter the genome. And yet, CRISPR-based methodologies continue to evolve. Recent publications demonstrate that CRISPR gene editing can be expanded in new directions to widen the utility and potential applications of this technology. The first presentation in this course will provide an overview of the latest developments in CRISPR-Cas-based techniques, with a focus on new Cas9 variants with new and expanded capabilities. The first presentation also will cover the new field of RNA targeting and the use of pooled CRISPR libraries with single cell transcriptomics to characterize complex phenotypes. The second presentation will focus on the use of CRISPR-Cas9-based screens from a toxicologist’s perspective. Specifically, this presentation will discuss how such screens can be used to gain a detailed, mechanistic understanding of a toxicant’s effect as well as the role of specific genes. Overall, this course is designed to provide an overview of the most recent advances in CRISPR-based technologies as well to provide some insight into future uses.
- Advances in CRISPR-Cas9 Tools and Applications for Toxicologists
Latest Advances in CRISPR-Cas Technologies.—E. Demireva. Michigan State University, East Lansing, MI. Sponsor: C. Rockwell
Use of CRISPR/Cas9-Based Genome-Wide Screens in Toxicology from a User’s Perspective.—C. Vulpe. University of Florida, Gainesville, FL.
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